Particle Size Determination using the Tyndall effect and Mie scattering theory

If a beam of converging rays, say, from a projector, is passed through a liquid containing minute particles in suspension, each of these particles scatters the light rays that fall on it, becoming, in a sense, a luminous point. Thus, the entire path of the rays through the liquid becomes visible. This gives the appearance of a bright cone when viewed in a darkened room. The Tyndall Effect can be seen in many examples in everyday life, such as headlight beams on foggy nights, when sunlight comes through a window into a dusty room, or comes down through holes in clouds. An example of the Tyndall effect is shown below in Fig 1.

image 02
Fig 1: This image shows the Tyndall effect. The first cuvette contains microspheres which reflect the light as it passes through, producing
a visible beam. The second cuvette just contains water with no particles suspended in it so the Tyndall effect does not occur

Scattering is a phenomenon in which the direction, frequency, or polarization of a wave is changed when the wave encounters discontinuities in a medium, or interacts with material at the atomic or molecular level Fig 1. It is a combination of reflection, refraction and diffraction. In the Tyndall effect the suspended particles act as the discontinuities in the medium which cause the light to be scattered. When the moment of the EM wave interacts with the particle, the electron orbits within the constituent molecules of the particle are perturbed periodically with the frequency of the electric field of the incident radiation. The oscillation or perturbation acts as a source of EM radiation resulting in a scattering of the incident light.

light scattered by a particle
Fig 2: scattering of light by a particle

 Mie scattering theory takes into account both absorbing and non-absorbing spherical particles without any particular constraints on particle size. Mie theory has no size limitations, can be used for any medium and converges to the limits of geometrical optics for large particles. The theory accurately predicts the angle and intensity of the light scattered by the light-particle interaction. 

The scattering pattern produced will result in a series of concentric bright and dark rings around a central maximum. The image of the scattering pattern is circularly symmetrical which means that the intensity of the scattered light will be equal in any direction for a given angle. This allows us to measure the scattering pattern in two dimensions, intensity and angle.


Fig 3. the scattering pattern produced by a red laser. Note the pattern is circularly symmetrical

How to find out  the size of  particles using mie scattering 
  1. First you will need a measurement system to record experimental data, a spectroscope is ideal for this as it already has an angular scale with a moveable arm.
  2. Next mount a  laser on the fixed arm of the spectroscope.
  3. On the moveable arm you will attach your photodetector. A photodiode sensitive to light of your lasers wavelength will be ideal for this.
  4.  Build a circuit for the photodiode to measure intensity of the light.    


    5.    The system is now capable of measuring  the intensity and angle of scattered light.
    6.    To record this data onto a computer you will need some sort of interface. A DAQ card will convert the output voltage to a digital signal and a program          
            will be needed to measure and record the values. I used LabView a data driven programming language for my system.
    7.    When all of this of this has been done your system  is ready to go. So the next step is measuring the size of  your microspheres.
    8.     A cuvette can be used to hold the sample. Place a small quantity of the spheres in the cuvette and fill with water to make a colloidal suspension.
    9.     Take measurements of the intensity of  the scattered light at different angles. Now you have your scattering pattern for the particles.
   10.    To find the size of these particles You can use a program called fitmie to compare your data to theoretical values and return the size of the particle that
             gave the best fit between your data and the theoretical data.
   11.    Your system should now be ready to measure the size of particles.



Here's one I made earlier: The photodetector is located in the tube on the left to block out background light.


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